Part:BBa_J70599:Design

VioA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1012
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacattcttccgatatctgcattgttg l: 59 P(g/b): g Ho(max#): -116.59 Het(max#): -116.59 Tm: 68.3 GC(%): 44.1

Reverse primer: gtttcttcctgcagcggccgctactagtattattacgcggcgatacgctgcaac l: 54 P(g/b): b Ho(max#): -112.7 Het(max#): -116.59 Tm: 70.7 GC(%): 53.7

Source

designed